Figure 7
From: Netrin-4 promotes mural cell adhesion and recruitment to endothelial cells
Netrin-4 induces mural cell coverage of tumor vessels in vivo. (A) Intravital microscopy of subcutaneous PC3 tumors (Control) and those overexpressing Netrin-4 (Netrin-4). The organization of blood vessels in Netrin-4 overexpressing tumors was better structured than in control tumors. Imaging probe field: 400 × 300 μm. (B) Desmin (red) and CD31 (green) immunostaining on tissue sections of four different tumors from Netrin-4 transfected (Netrin-4) and empty vector transfected PC3 (Control) groups. Fields were examined by immunofluorescence microscopy, data were analyzed by Image J software and results expressed as mean pixels per μm2 (20 fields per condition). A Student’s t test was used to assess the statistical significance of differences between groups *P < 0.05. Desmin staining was significantly greater in Netrin-4-overexpressing tumors (graph in B) and co-localized with CD31 immunostaining (merge: Desmin/CD31) indicating that Netrin-4 promotes VSMC coverage of tumor vessels.