Figure 6
From: Netrin-4 promotes mural cell adhesion and recruitment to endothelial cells
Netrin-4 enhances VSMC recruitment on HUAEC in vitro. (A) Co-culture of VSMC (stained in red) and HUAEC (stained in green) grown on Matrigel indicated that pericyte coverage was greater with VSMC pretreated with Netrin-4 (N4) compared with non-treated cells (Control). (B) Silencing of Netrin-4 expression in VSMC using siRNA significantly reduced HUAEC coverage by VSMC (arrows). RT-PCR analysis showed that N4 expression is down-regulated in VSMC transfected with Netrin-4 siRNA (N4siRNA), but not in non-transfected (NT) cells or in VSMC transfected with a control siRNA (Control siRNA). A Student’s t test was used to assess the statistical significance of differences between groups **P < 0.01, *P < 0.05.