Figure 7
From: Notch and VEGF pathways play distinct but complementary roles in tumor angiogenesis
Silencing of Notch1 does not recapitulate the vasculature seen with NGP-N1D. (A) NGP-EV and NGP-shN1 tumor weights were not different. Error bars represent SD. (B) The number of TUNEL positive cells is not different between the NGP-EV and NGP-shN1 tumors. (C) Cleaved Notch1 is present in vasculature of NGP-EV and NGP-shN1 (arrows). (D) The EC marker PECAM-1 does not show discontinuity or lack of coverage in vasculature of NGP-shN1 tumors. Bar = 100 μm. (E) The percentage of PECAM-1 coverage did not differ between NGP-EV and NGP-shN1 tumors. Error bars represent SD. P = n.s. (F) The pericyte marker αSMA does not show erratic, discontinuous immunostaining in NGP-shN1 tumors. Bar = 100 μm. (G) There was no difference in percentage of αSMA coverage between NGP-EV and NGP-shN1 tumors. Error bars represent SD. P = n.s.