Figure 3
From: Notch and VEGF pathways play distinct but complementary roles in tumor angiogenesis
Blockade of VEGF increases tumor hypoxia. (A) Immunostaining for pimonidazole hypoxyprobe shows that NGP-LacZ controls (top left) show almost no detectable staining. Both NGP-N1D and NGP-LacZ + BV (top right and bottom left) show increased areas of Hypoxyprobe in some areas of the tumor, but wide areas remained well oxygenated. NGP-N1D + BV tumors (bottom right) show Hypoxyprobe staining throughout the tumor. Bar = 400 μm. (B) Quantification of Hypoxyprobe staining showed increased the hypoxia of NGP-LacZ + BV and NGP-N1D + BV compared to NGP-LacZ controls. Error bars represent SD. *P < 0.05, **P < 0.001. (C) VEGF expression was increased in NGP-LacZ + BV and NGP-N1D + BV compared to NGP-LacZ or NGP-N1D. Error bars represent SD. **P < 0.01, ***P < 0.001. (D) GSEA enrichment plots are shown for HARRIS_HYPOXIA gene set NGP-N1D + BV vs NGP-LacZ, and for SEMENZA_HIF1_TARGETS NGP-LacZ + BV vs NGP-LacZ. Normalized Enrichment Score (NES) and False discovery rated (FDR) q-values are shown.