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Figure 3 | Vascular Cell

Figure 3

From: Two distinct types of the inhibition of vasculogenesis by different species of charged particles

Figure 3

Distinct modifications of the matrix architecture. 24 hours after plating 3- Dimensional cultures of HBMEC were either unexposed A or exposed to 1Gy Fe ions B and 1 Gy protons C, then cultured for a further 5 days before fixation. Two-photon microscopy was used to visualize cell material stained with DTAF and collagen was visualized by Second Harmonic Generation microscopy (as shown). Control cultures have branched vessels with compressed bright staining collagen deposits surrounding them. Cultures exposed to Fe ions B display narrow guidance tunnels (arrow) surrounding narrow processes. Cultures exposed to protons C display shorter widened tunnels lined with compressed bright staining collagen deposits (Arrowhead). Bar = 50 μm.

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