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Figure 3 | Vascular Cell

Figure 3

From: Arteriogenic therapy based on simultaneous delivery of VEGF-A and FGF4 genes improves the recovery from acute limb ischemia

Figure 3

VEGF-A gene transfer stimulates neovascularization of normo-perfused mouse adductor muscles. (A) Capillary density quantified at different time-points after AAV-mediated muscle transduction. (B) Representative pictures of FITC-lectin-stained (green) capillaries 21 days after AAV-mediated gene transfer (left panel) and thigh muscle tissue histology (H&E) at day 28 following injection with different AAV (right panel). Magnification 200x and 400x, respectively. (C) Proliferating PCNA-positive capillary ECs in normo-perfused adductors 28 days after transduction. (D) Representative pictures of the double immunofluorescent staining detecting biotinylated lectin B4 Bs bound to ECs (red) and PCNA (green) in cells undergoing DNA synthesis. Double positive cells are indicated by arrows. DAPI was used to stain the nuclei (blue) and to confirm the nuclear localization of PCNA. Magnification 1000x. Values are means ± SEM (n = 3/group and time-point), * p < 0.05 vs AAV-LacZ. # p < 0.05 vs AAV-VEGF-A. $ p < 0.05 vs, appropriately, AAV-VEGF-A or AAV-FGF4-IRES-VEGF-A at day 21.

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Vascular Cell

ISSN: 2045-824X

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