Primers | Sequences (5’-3’) | Product size (bp) | Annealing T (°C) |
---|---|---|---|
Human | |||
CD31-f | GCT GTT GGT GGA AGG AGT GC | 645 | 49 |
CD31-r | GAA GTT GGC TGG AGG TGC TC | ||
vWF-f | CAC TGA CAC CTG AGT GAG AC | 696 | 60 |
vWF-r | GTT CGT CCT GGA AGG ATC GG | ||
Tie-2-f | GGC CGC TAC CTA CTA ATGA AG | 550 | 50 |
Tie-2-r | CGT GAT TGA CAC TGG ACA TAA | ||
P-gp-f | GCA AAG CTG GAG AGA TCC TCA CCA | 305 | 60 |
P-gp-r | CAA CAT TTT CAT TTC AAC AAC TCC TGC | ||
GAPDH-f | CGG AGT CAA CGG ATT TGG TCG TAT | 307 | 58 |
GAPDH-r | AGC CTT CTC CAT GGT GGT GAA GAC | ||
Mouse | |||
CD31-f | GTC ATG GCC ATG GTC GAG TA | 260 | 55 |
CD31-r | CTC CTC GGC GAT CTT GCT GAA | ||
vWF-f | TGT TCA TCA AAT GGT GGG CAG C | 270 | 62 |
vWF-r | ACA GAC GCC ATC TCC AGA TTC A | ||
Tie-2-f | GGA CAG TGC TCC AAC CAA ATG | 154 | 60 |
Tie-2-r | GAC GGA AAT GTT GAA AGG C | ||
P-gp-f | TGC TTA TGG ATC CC | 435 | 55 |
P-gp-r | TTG GTG AGG ATC TCT CCG GCT | ||
GAPDH-f | GTC GGT GTG AAC GGA TTT G | 280 | 55 |
GAPDH-r | TAG ACT CCA CGA CAT ACT CAG CA |