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Table 3 RT-PCR primer sequences and expected product size

From: Human and mouse brain-derived endothelial cells require high levels of growth factors medium for their isolation, in vitro maintenance and survival

Primers Sequences (5’-3’) Product size (bp) Annealing T (°C)
Human    
CD31-f GCT GTT GGT GGA AGG AGT GC 645 49
CD31-r GAA GTT GGC TGG AGG TGC TC   
vWF-f CAC TGA CAC CTG AGT GAG AC 696 60
vWF-r GTT CGT CCT GGA AGG ATC GG   
Tie-2-f GGC CGC TAC CTA CTA ATGA AG 550 50
Tie-2-r CGT GAT TGA CAC TGG ACA TAA   
P-gp-f GCA AAG CTG GAG AGA TCC TCA CCA 305 60
P-gp-r CAA CAT TTT CAT TTC AAC AAC TCC TGC   
GAPDH-f CGG AGT CAA CGG ATT TGG TCG TAT 307 58
GAPDH-r AGC CTT CTC CAT GGT GGT GAA GAC   
Mouse    
CD31-f GTC ATG GCC ATG GTC GAG TA 260 55
CD31-r CTC CTC GGC GAT CTT GCT GAA   
vWF-f TGT TCA TCA AAT GGT GGG CAG C 270 62
vWF-r ACA GAC GCC ATC TCC AGA TTC A   
Tie-2-f GGA CAG TGC TCC AAC CAA ATG 154 60
Tie-2-r GAC GGA AAT GTT GAA AGG C   
P-gp-f TGC TTA TGG ATC CC 435 55
P-gp-r TTG GTG AGG ATC TCT CCG GCT   
GAPDH-f GTC GGT GTG AAC GGA TTT G 280 55
GAPDH-r TAG ACT CCA CGA CAT ACT CAG CA