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Table 3 RT-PCR primer sequences and expected product size

From: Human and mouse brain-derived endothelial cells require high levels of growth factors medium for their isolation, in vitro maintenance and survival

Primers

Sequences (5’-3’)

Product size (bp)

Annealing T (°C)

Human

   

CD31-f

GCT GTT GGT GGA AGG AGT GC

645

49

CD31-r

GAA GTT GGC TGG AGG TGC TC

  

vWF-f

CAC TGA CAC CTG AGT GAG AC

696

60

vWF-r

GTT CGT CCT GGA AGG ATC GG

  

Tie-2-f

GGC CGC TAC CTA CTA ATGA AG

550

50

Tie-2-r

CGT GAT TGA CAC TGG ACA TAA

  

P-gp-f

GCA AAG CTG GAG AGA TCC TCA CCA

305

60

P-gp-r

CAA CAT TTT CAT TTC AAC AAC TCC TGC

  

GAPDH-f

CGG AGT CAA CGG ATT TGG TCG TAT

307

58

GAPDH-r

AGC CTT CTC CAT GGT GGT GAA GAC

  

Mouse

   

CD31-f

GTC ATG GCC ATG GTC GAG TA

260

55

CD31-r

CTC CTC GGC GAT CTT GCT GAA

  

vWF-f

TGT TCA TCA AAT GGT GGG CAG C

270

62

vWF-r

ACA GAC GCC ATC TCC AGA TTC A

  

Tie-2-f

GGA CAG TGC TCC AAC CAA ATG

154

60

Tie-2-r

GAC GGA AAT GTT GAA AGG C

  

P-gp-f

TGC TTA TGG ATC CC

435

55

P-gp-r

TTG GTG AGG ATC TCT CCG GCT

  

GAPDH-f

GTC GGT GTG AAC GGA TTT G

280

55

GAPDH-r

TAG ACT CCA CGA CAT ACT CAG CA