Table 2 Human and mouse antibodies utilized for immunofluorescence and flow cytometry analyses
Antibody | Dilution | Manufacturer |
|---|---|---|
Immunofluorescence | ||
Human | ||
CD31 | 1:50 | Sigma |
vWF | 1:50 | Sigma |
GLUT-1 | 1:100 | Millipore |
e-NOS | 1:100 | Millipore |
UEA-1 | 1:20 | Sigma |
hNAg | 1:20 | Chemicon |
Draq 5 | 1:800 | Alexis |
VE-cadherin | 1:250 | Santa Cruz |
Claudin-5 | 1:1000 | Invitrogen |
β-catenin | 1:250 | Abcam |
Mouse | ||
CD31 | 1:100 | Millipore |
vWF | 1:50 | Santa Cruz Biotechnology |
GLUT-1 | 1:100 | Millipore |
e-NOS | 1:100 | Millipore |
Flow cytometry | ||
Human | ||
CD31-PE (AC128) | 1:40 | Miltenyi |
CD105-FITC ( SN6) | 1:40 | Serotec |
CD146-PE ( F4-35H7) | 1:20 | Biocytex |
UEA-1-FITC | 1:50 | Sigma |
Mouse | ||
CD31-PE (MEC13.3) | 1:20 | BD Biosciences |
CD34-FITC (RAM34) | 1:20 | BD Biosciences |
CD146-FITC (P1H12) | 1:20 | Chemicon |
Tie-2-PE (1E11DH) | 1:50 | Millipore |