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Figure 4 | Vascular Cell

Figure 4

From: Human and mouse brain-derived endothelial cells require high levels of growth factors medium for their isolation, in vitro maintenance and survival

Figure 4

Gene expression profile and in vivo engraftment. A) RT2 profiler of human BMVECs cultured in EndoPM in comparison with cells cultivated in complete EBM; B) Endothelial gene expression of human and murine BMVECs analyzed by means of qualitative PCR. C-D) in vivo engraftment: photographs of the abdominal quadrant of CD1 mice that received C) or did not receive D) human BMVECs cultured in EndoPM; E) Grafted human BMVECs abdominal quadrant is used for immunostaining. Cells were immunoreactive for UEA-1 (green) 2 weeks after transplantation. The presence of live human ECs was confirmed by Draq5 (red) positivity and anti-human nuclei (hNAg, blue) positivity. The lower-right image represents the merge between UEA-1, Draq5 and anti-human nuclei. Scale bar, 50 μm. F) The control abdominal quadrant that received vehicle only resulted UEA-1 negative (upper-left image) and Draq5 (red) positive. DAPI was used for nuclear staining. The lower-right image represents the merge between UEA-1, Draq5 and DAPI. Scale bar, 20 μm.

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Vascular Cell

ISSN: 2045-824X

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