Figure 1From: Human and mouse brain-derived endothelial cells require high levels of growth factors medium for their isolation, in vitro maintenance and survivalCulture characteristics of human and murine BMVECs. A) Growth curve of human and mouse BMVECs cultured in EndoPM (black line) or complete EBM (gray lines); B) proliferation index of human and mouse BMVECs cultured in EndoPM (black bars) or EBM (gray bars) at 7 DIV; C) survival index of human and mouse BMVECs cultured in EndoPM (black bars) or EBM (gray bars) 12 hours after culture; D-G) cell cycle profiles of human and mouse BMVEC growth in EBM (D, F) or EndoPM (E, G). Histograms are representative of one of four independent experiments. H) Co-culture of NHDF and BMVECs in EndoPM (black bars) or EBM (gray bars) after 0, 7 and 14 days of culture. Bars represent the number of CFDA-SE positive cells (NHDF) per field (Higer power field - HPF). * P < 0.05; **P < 0.01; ***P < 0.001.Back to article page