Figure 4

Diffusion pattern of heparin through various layer of CAM. CAM treated 1X PBS doesn’t show any diffusion pattern (a) stained without HRP and (b) with HRP. CAM treated with 50 μM heparin shows diffusion at chorionic (ce) and sub epithelial layers (c) stained without HRP and (d) with HRP. CAM treated with 100 μM heparin shows diffusion at ce and sub epithelial layers and also at stroma region (e) stained without HRP and (f) with HRP. CAM treated with 150 μM heparin shows higher intensity of HRP staining due to higher concentration and shows diffusion pattern at ce and sub epithelial layers and also at stroma region (g) stained without HRP and (h) with HRP. Arrow indicates the presence of biotin-streptavidin complex. Magnification for a, c, e and g is 10X and for b, d, f and h is 40X and are the results of 3 different set of experiments. For a, c, e and g bar is 50 μm and for b, d, f and h bar is 100 μm.