Figure 2

Effect of heparin on CAM morphology. (a) CAM treated with 1X PBS shows thin chorionic (ce) and allantoic (ae) epithelial layer due to minimal accumulation of fibroblast cells (fb) with subepithelial capillary network (SEC) along with the presence of large blood vessels (lbv) and vein (v). (b) CAM treated with 50 μM heparin shows increased thickness at ce and ae due to fb accumulation along with the presence of small blood vessels (sbv) at stroma region. (c) CAM treated with 100 μM heparin shows increased thickness at the ae and ce due to increased accumulation of fb with densely packed small blood vessels beneath SEC and also ECs capillary sprouting as small sprouting vessels (sv) which extending towards SEC. Presence of numerous small capillaries (sc) and lbv along with v are also visible at the stroma region. (d)150 μM heparin shows diminished fibroblast accumulation at thin stratum and also reiteration of radial arrangement of fb, lbv and sbv are also visible with minimal sprouting of sc. Gelatin sponges did not cause wound or inflammation. Representative tissues are 7 μm in thickness stained with haematoxylin and eosin and images were taken at 40X magnification and are the result of 3 different set of experiments. Bar is 50 μm.