Figure 1

Detection of endogenous PTN and RPTPβ/ζ in the chicken embryo CAM. A. Equal amounts of protein extracts of chicken embryo CAM from different developmental stages were analyzed by SDS-PAGE, followed by Western blot analysis for PTN and actin. The protein amounts were quantified by densitometric analysis of the corresponding bands and the ratio PTN/actin was calculated for each lane. B. Products of RT-PCR reactions for chicken PTN and GAPDH from mRNA of chicken embryo CAM from different developmental stages. The mRNA amounts were quantified by densitometric analysis of the corresponding bands and the ratio PTN/GAPDH was calculated in each lane. Results in both A and B are expressed as mean ± S.E.M. of the % change of the PTN relative amounts compared to day 6. C. Three mg of total protein from chicken embryo CAM extracts from different developmental stages were subjected to immunoprecipitation for RPTPβ/ζ. Precipitated proteins were analyzed by SDS-PAGE, followed by Western blot for the presence of RPTPβ/ζ. D. Three mg of total protein from chicken embryo CAM extracts from different developmental stages were subjected to immunoprecipitation for RPTPβ/ζ. Precipitated proteins were analyzed by SDS-PAGE, followed by Western blot analysis for the presence of PTN. The protein amounts in C and D were quantified by densitometric analysis of the corresponding bands and results are expressed as mean ± S.E.M. of the % change compared to day 6. Asterisks in all cases denote a statistically significant difference from day 6. **P < 0.01, ***P < 0.001.