Figure 4

HB-19 signalling down-regulates pSRC, pFAK, pAKT and pERK1/2. Western blot analysis of phosphorylated SRC, FAK, AKT and ERK1/2, in cells stimulated by increasing concentrations of HB-19 for 15 minutes. The blots were stripped and re probed for total SRC, HSC70, HSC70 and total ERK1/2 respectively. Results are expressed as % change relative to the control and are mean values ± SE from at least 3 independent experiments.