Figure 3

HB-19 down-regulates MMP2 in HUVECs. (A) Endothelial cells were cultured in a minimal medium with increasing concentrations of HB-19 and 8 h later the supernatants were analyzed for MMP2 activity by zymography. Results are expressed as % change relative to control and are mean values ± SE from at least 3 independent experiments. (B) Endothelial cells were incubated with increasing concentrations of HB-19 and 24 h later total RNA was extracted from the cells, RT-PCR reactions were performed using specific primers for MMP2 or GAPDH mRNAs, the PCR products were analyzed in agarose gels and quantified. Results are expressed as % change relative to control and are mean values ± SE from at least 3 independent experiments.