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Figure 1 | Vascular Cell

Figure 1

From: The pseudopeptide HB-19 binds to cell surface nucleolin and inhibits angiogenesis

Figure 1

HB-19 inhibits the in vitro adhesion, proliferation, migration and motility of HUVECs. (A) Inhibition of HUVECs adhesion by HB-19. An equal number of HUVECs were incubated with increasing concentrations of HB-19 for 30 min before seeding. After a 45 min incubation period, adherent cells were measured by the crystal violet assay. Results are expressed as % change relative to control and are mean values ± SE from at least 3 independent experiments. (B) Inhibition of HUVECs proliferation by HB-19. Cells were cultured for 3 days in presence of increasing concentrations of HB-19. Cell proliferation was quantified by crystal violet staining. Results are expressed as % change relative to control and are mean values ± SE from at least 3 independent experiments. (C) Migration of cells through Transwell filters. The lower compartment of Transwell filters (8 μm pores) was filled with growth media containing 0.25% BSA. An equal number of HUVECs was re suspended in growth medium containing 0.25% BSA and increasing concentrations of HB-19, and transferred into Transwell inserts. Cells that successfully migrated through the filter pores, were fixed, stained and quantified by counting the entire area of each filter. Results are expressed as % change relative to control and are mean values ± SE from at least 3 independent experiments. (D) Confluent cell monolayers were scratched and cells were left to heal the wound in the presence of increasing concentrations of HB-19. 48 h later the plates were photographed.

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