Figure 1

The effect of ARPs on VRI-induced blood vessel loss in Tg(fli-1a:EGFP)y1 zebrafish (result of morphological observation). White arrows indicate DLAV, ISV and DA of zebrafish. Scale bar = 500 μm. (A-A') Control group: 24 hpf embryos were treated with 0.1% DMSO for 24 h and 48 h. 24 hpf embryos were treated with VRI (300 nM) at for 3 h. After that, the VRI was washed out and replaced with 0.1% DMSO (v/v) embryo medium (B-B') or 300 μg/ml P1 (C-C'), 100 μg/ml P2 (D-D'), 300 μg/ml P3 (E-E'), 10 μg/ml (F-F'), 30 μg/ml (G-G') and 100 μg/ml (H-H') P4, 30 μg/ml (I-I'), 100 μg/ml (J-J') and 300 μg/ml (K-K') P5 for 24 h.