Figure 2From: Cooperative benefit for the combination of rapamycin and imatinib in tuberous sclerosis complex neoplasiablot analysis using Akt and pAkt were conducted on Tsc2 ang1 cells treated with treated with DMSO, rapamycin5nM, imatinib10uM or rapamycin5nM + imatinib10uM for 24 hours. Cells were lysed and analyzed by using antibodies specific for the total Akt and pAkt473. Rapamycin + imatinib treatment downregulates pAkt levels (p <0.0002). GAPDH was used as the loading control by using monoclonal anti-GAPDH antibody. All treatment groups were significant when compared to the vehicle control, Imatinib 10nM (p < 0.0006); Rapamycin 5nM (p < 0.0076); Rapamycin 5nM + Imatinib 10nM (p < 0.0002). However, the combinational therapy only show significance against Rapamycin at 5nM (p < 0.0243) and not Imatinib at 10nM (p < 0.4273).Back to article page