Figure 6From: The role of AMP-activated protein kinase in the functional effects of vascular endothelial growth factor-A and -B in human aortic endothelial cellsThe effect of VEGF on fatty acid oxidation and uptake. A) Fatty acid oxidation was assessed in HAECs incubated in the presence or absence of the indicated concentrations of VEGF-A, VEGF-B or AICAR for 4 h. B) HAECs were incubated in the presence or absence of VEGF-A (25 ng/ml) or VEGF-B (250 ng/ml) for 24 h and fatty acid uptake assessed. Ad.control, Ad.AMPK-DN and Ad.AMPK-CA adenoviruses were used to infect HAECs 24 h prior to the addition of VEGF. C) HAECs were incubated in the presence or absence of VEGF-A (25 ng/ml) and L-NAME (1 mM) for 24 h and fatty acid uptake assessed. Results are expressed as A) the mean ± SEM basal fatty acid oxidation or B,C) the mean ± SEM basal fatty acid uptake for 3 independent experiments performed in triplicate. *p < 0.05 relative to Ad.control-infected HAECs. **p < 0.05 relative to vehicle.Back to article page