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Figure 6 | Vascular Cell

Figure 6

From: The role of AMP-activated protein kinase in the functional effects of vascular endothelial growth factor-A and -B in human aortic endothelial cells

Figure 6

The effect of VEGF on fatty acid oxidation and uptake. A) Fatty acid oxidation was assessed in HAECs incubated in the presence or absence of the indicated concentrations of VEGF-A, VEGF-B or AICAR for 4 h. B) HAECs were incubated in the presence or absence of VEGF-A (25 ng/ml) or VEGF-B (250 ng/ml) for 24 h and fatty acid uptake assessed. Ad.control, Ad.AMPK-DN and Ad.AMPK-CA adenoviruses were used to infect HAECs 24 h prior to the addition of VEGF. C) HAECs were incubated in the presence or absence of VEGF-A (25 ng/ml) and L-NAME (1 mM) for 24 h and fatty acid uptake assessed. Results are expressed as A) the mean ± SEM basal fatty acid oxidation or B,C) the mean ± SEM basal fatty acid uptake for 3 independent experiments performed in triplicate. *p < 0.05 relative to Ad.control-infected HAECs. **p < 0.05 relative to vehicle.

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