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Figure 1 | Vascular Cell

Figure 1

From: The role of AMP-activated protein kinase in the functional effects of vascular endothelial growth factor-A and -B in human aortic endothelial cells

Figure 1

VEGF-B stimulates AMPK in HAECs. HAECs were incubated in the presence or absence of A-C) the indicated concentrations of VEGF-A or VEGF-B for 5 min or D) 100 ng/ml VEGF-B for the durations indicated and lysates prepared. Lysates were subsequently subjected to A) assay of AMPK activity or B,C,D) immunoblotting with the antibodies indicated. A) The results are expressed as the mean ± SEM% basal AMPK activity for four independent experiments. B,D) Representative immunoblots are shown, repeated with similar results on three independent sets of lysates. C) Quantification of immunoblots. *p < 0.05 relative to value in absence of VEGF, **p < 0.01 relative to value in absence of VEGF.

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Vascular Cell

ISSN: 2045-824X

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