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Figure 7 | Vascular Cell

Figure 7

From: Changes in expression of VE-cadherin and MMPs in endothelial cells: Implications for angiogenesis

Figure 7

Changes in the production and distribution of VE Cadherin and β-catenin in HUVECs: Effect of curcumin. (A) Production of VE Cadherin-Western blotting: HUVECs were maintained in culture in MCDB 131 medium in presence of curcumin (10 μM) for different time intervals. Medium was removed, the cells were harvested and lysed in Laemmeli buffer and protein equivalent amount of the cell lysates normalized to the total cell protein were subjected to SDS PAGE (bottom) and western blot (top) analysis with anti VE-cadherin antibody as described in the text. Lanes 1-4 represents 1st -4th day (A). The intensity of the immunoblotted bands were measured and expressed in intensity units/mm2/mg protein. Values for control in intensity units/mm2 (Day 1: 1256 ± 145, Day 2: 2678 ± 156, Day 3: 2287 ± 189, Day 4: 4018 ± 178) * Statistically significant when compared to the production on first day p < 0.05. (B) Changes in the distribution of β-catenin in HUVECs HUVECs were maintained in culture in presence of curcumin for (10 μM) for different time intervals as above. The cells were harvested and nuclear and cytosol were isolated. Protein equivalent amount of the cytosolic and nuclear fractions were subjected to SDS PAGE and western blot analysis with anti β-catenin antibody as described in the text. Values for control in intensity units/mm2/mg protein (Nucleus Day 1: 1877 ±, 145 Day 2: 1687 ± 102, Day 3: 987 ± 89, Day 4: 417 ± 96 and Cytosol Day 1: 0, Day 2: 0, Day 3: 887 ± 69, Day 4: 1567 ± 58). Lane1-4 1st -4th day (inset). The intensity of the immunoblotted bands were measured and expressed in intensity units/mm2/mg protein (a). Percentage distribution of β-catenin in the nucleus and cytosol was calculated and expressed in the percentage bar diagram (b). Values for control (Nucleus Day 1: 100, Day 2: 100, Day 3: 52, Day 4: 21 and Cytosol Day 1: 0, Day 2: 0, Day 3: 48, Day 4: 79). * Statistically significant when compared to their relative levels in nucleus and cytosol on first two days p < 0.05.

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