Figure 2
From: Changes in expression of VE-cadherin and MMPs in endothelial cells: Implications for angiogenesis
Expression of MMP-2 and MMP-9 in HUVECs - RT-PCR: Cells were maintained in MCDB 131 medium for different time intervals. Medium was removed and the cell layer harvested, total RNA was isolated and subjected to RT-PCR as described in the text. Samples were resolved on 1.75% agarose gel MMP-2 (A) and MMP-9 (B). Intensity of bands were quantitated and normalized with the intensity of band for internal control GAPDH and expressed as intensity/mm2 (C). * Statistically significant when compared to their production on first day p < 0.05.