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Figure 6 | Vascular Cell

Figure 6

From: Complementary effects of extracellular nucleotides and platelet-derived extracts on angiogenesis of vasa vasorum endothelial cells in vitro and subcutaneous Matrigel plugs in vivo

Figure 6

Quantitative analysis of tube formation in nucleotide-containing Matrigel plugs. Data in panels A-C represent means ± SE for the amount of tubes detected in right- and left-side placed plugs for each animal in the group. Five to ten random fields were analyzed per each section. (A): Tube formation in control and platelet extract containing Matrigel plugs (concentrations as indicated). Total number of tubes per animal group were use for statistical analysis of variances; p > 0.05 and p < 0.05 vs. control group; (B): Tube formation in Matrigel plugs containing ATP or mixtures of MeSATP, ATPγS, MeSADP, and ADPβS (concentrations as indicated). Total number of tubes per animal group were use for statistical analysis of variances; p > 0.05 vs. control group, shown in panel A); (C): Tube formation in Matrigel plugs containing platelet extracts and mixtures of MeSATP, ATPγS, MeSADP, and ADPβS (concentrations as indicated); *p < 0.05 vs. nucleotides alone, **p < 0.01 vs. nucleotides alone); (D): Summary of the analysis of tube formation for all experimental conditions. Data are mean ± SE for the total amount of tubes for each group; *p < 0.05 vs. control; # p < 0.05 vs. nucleotides alone; # # p < 0.01 vs. nucleotides alone; (E): Quantitative analysis of functional capillaries in Matrigel plugs. The capillaries of at least 50 microns from a native tissue edge were considered newly created within the plug and were not part of those preexistent in the native tissue. Five to ten random fields were analyzed per each section. Data are means ± SE (n = 5) for each condition; *p < 0.05 vs. control; **p < 0.01 vs. control # p < 0.05 vs. nucleotides alone; # # p < 0.01 vs. nucleotides alone.

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