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Figure 3 | Vascular Cell

Figure 3

From: Complementary effects of extracellular nucleotides and platelet-derived extracts on angiogenesis of vasa vasorum endothelial cells in vitro and subcutaneous Matrigel plugs in vivo

Figure 3

Platelet extracts and extracellular ATP elicit VVEC rearrangements into tube-like network on Matrigel. (A): Growth-arrested VVEC (1.25 × 105 cells/well) were plated on growth factor-reduced Matrigel in serum free DMEM. Formation of tube-like networks was stimulated by the addition of platelet extracts (16 μg/ml), ATP (100 μM), or combination of both. After incubation, images were captured in three fields using Nikon microscope connected with digital camera. The data show one representative image for each experimental conditions; arrows indicate cells visible within the Matrigel; (B): Quantitative evaluation of tube formation using S.CORE Image Analysis. Data represent the means ± SEM of three independent experiments; # p < 0.05, # # p < 0.05, vs. nonstimulated control (total length of poorly-developed tubes), * p < 0.05, *** p < 0.001 vs. nonstimulated control (total length of satisfactory-developed tubes), § p < 0.05 vs. nonstimulated control (total length of well-developed tubes).

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Vascular Cell

ISSN: 2045-824X

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