Figure 9

The effect of hypoxia HRPC-CM on level and distribution of HIF-1α. (A) HRPC cells were cultured with normoxia HUVEC-CM (B) HRPC cells were cultured with hypoxia HUVEC-CM. Cells were analyzed by immunofluorescence with primary antibody that recognize the HIF-1α and followed by secondary antibody FITC- conjugated IgG. Cells were counterstained with DAPI (blue) to label nuclei and analyzed using fluorescence microscopy. (C) Western blots analysis of HIF-1α in cytoplasmic and nuclear fractions were obtained from the HRPC cells were cultured with normoxia/hypoxia HUVEC-CM. (D) Densitometry quantification of signal intensities of HIF-1α and β-actin. Results are representatives of three independent experiments.