Figure 7

RT-PCR and Western blot analysis of HIF-1α. Total RNA and total protein were extracted from HRPC and HUVEC cultured alone or co-cultured under normoxia and hypoxia conditioned for 24 h. The expression of HIF-1α was measured by (A) electrophoresis of RT-PCR, (C) Western blot analysis in the HRPC and HUVEC. Figures (B, D) the band intensities corresponding to the HIF-1α were quantified by image analysis using a Bio-Rad scanning densitometer and Quantity One analysis software. Data were shown as ratio of HIF-1α densities to that of 18S RNA for RT-PCR and β-actin antibody was used to normalize Western blot for differences in loading and the transfer efficiencies. All data were expressed as mean +/- SE of three independent experiments.