Figure 4

The effects of hypoxia and co-cultured of HRPC and HUVEC on VEGF secretion. Both HRPC and HUVEC were cultured singly and as co-cultures under normoxia (■) and hypoxia (□) conditions for specified time and medium were collected as described in Material and Methods. The secreted VEGF was determined by ELISA as described in above. The values presented as bar graphs give the means ± S.E.M. of quadruplicate from three independent experiments. The effects of both cells cultured/co-cultured on the secretion of endogenous VEGF protein are statistically significant under hypoxia conditions. Data shown is mean ± S.E.M. of three independent experiments (n = 4), *P>0.001.