3A; Laser Doppler perfusion Images, Transfixation group; Pre-operative, Post-Operative, Day 12, Day 24 and Day 42 post ischemia (1,2,3,4 and 5 respectively). 3B; Mean Difference in perfusion, Transfixation group. Figure 3C; Skin from medial aspect of left thigh with normal appearance of dermis, epidermis and skin appendages (1); Left thigh with normal appearance of skeletal muscle fibers (2); Left calf with normal appearance of skeletal muscle fibers (3); Skin from medial aspect of right thigh with mild vascular congestion and mild inflammatory cellular infiltration (4); Right thigh with normal appearance of skeletal muscle fibers (5); Right calf with vascular congestion, damage to some skeletal muscle fibers and replacement by adipose tissue (6). All sections were taken at 200 ×, stained with Hematoxylin and Eosin stain. Scale bars are 100 μm.