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Figure 6 | Vascular Cell

Figure 6

From: Low doses of 3-aminobenzamide, a poly(ADP-ribose) polymerase inhibitor, stimulate angiogenesis by regulating expression of urokinase type plasminogen activator and matrix metalloprotease 2

Figure 6

Effect of MMP-2 inhibition on in vitro tubulogenesis. (a) Representative photographic fields showing the effect of MMP-2 inhibition on in vitro tubulogenesis of HUVEC treated for 4 h with FGF2 (10 ng/ml) in the presence of 3ABA (50 μM), of 3ABA plus Ilomastat (25 μM), of 3ABA plus anti-MMP-2 antibody (10 μg/ml). (b) Quantification of tubulogenesis. Results were quantified by measuring the percent field occupancy of tubules. FGF2-stimulated HUVEC treated with 3ABA decreased the tube formation and the length of the vascular network when supplemented with MMP-2 inhibitor Ilomastat and anti-MMP-2 antibody. Values are mean ± SEM of six experiments. The percent decrease/increase of tubulogenesis is referred to FGF2 plus 3ABA treated cells taken as 100%. Multiple comparisons were performed by the Student-Newman-Keuls test, after Kruskal-Wallis analysis. (# p < 0.05 compared to FGF2 plus 3ABA treated cells).

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Vascular Cell

ISSN: 2045-824X

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