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Figure 4 | Vascular Cell

Figure 4

From: Low doses of 3-aminobenzamide, a poly(ADP-ribose) polymerase inhibitor, stimulate angiogenesis by regulating expression of urokinase type plasminogen activator and matrix metalloprotease 2

Figure 4

Effect of 3ABA on uPA and uPAR gene expression. (a) Representative experiment of uPA and uPAR gene expression analyzed in HUVEC untreated (Control) and treated with FGF2 (10 ng/ml), with FGF2 plus 3ABA (50 μM), and with 3ABA, for 24 h. cDNAs were serially diluted and amplified by PCR using specific primers for uPA, uPAR gene or GAPDH as a control housekeeping gene. Cells exposed to FGF2 for 24 h showed an increase in uPA-mRNA compared to control cells, while exposure to 3ABA inhibited FGF2-stimulated uPA gene expression. No difference in uPAR-mRNA was found. (b) Quantification of uPA and, uPAR gene expression. Values are expressed as ratio between normalized integrated densities of uPA, uPAR and GAPDH PCR products, and are mean ± SEM of three experiments. Multiple comparisons were performed by the Student-Newman-Keuls test, after Kruskal-Wallis analysis. (* p < 0.05 compared to control; # p < 0.05 compared to FGF2 treated cells).

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Vascular Cell

ISSN: 2045-824X

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