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Figure 2 | Vascular Cell

Figure 2

From: Low doses of 3-aminobenzamide, a poly(ADP-ribose) polymerase inhibitor, stimulate angiogenesis by regulating expression of urokinase type plasminogen activator and matrix metalloprotease 2

Figure 2

Effect of 3ABA on in vitro tubulogenesis. (a) Representative photographic fields showing the effect of 3ABA on in vitro tubulogenesis. HUVEC tubulogenesis assay was performed in the absence (Control), in the presence of FGF2 (10 ng/ml), of FGF2 plus 3ABA (50 μM), and of 3ABA. Pictures were taken after 4 h. HUVEC stimulated with FGF2 started to differentiate in tube-like structures. Exposure to 3ABA of FGF2-stimulated cells enhanced tube formation with a rise of the number of interconnections, and the thickness of tubules. (b) Quantification of tubulogenesis after 3ABA treatment. Results were quantified by measuring the percent field occupancy of tubules. Values are mean ± SEM of six experiments. The percent decrease/increase of tubulogenesis is referred to control untreated cells taken as 100%. Multiple comparisons were performed by the Student-Newman-Keuls test, after Kruskal-Wallis analysis. (* p < 0.05 compared to control; # p < 0.05 compared to FGF2 treated cells).

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Vascular Cell

ISSN: 2045-824X

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