Figure 6
From: A deficiency of uPAR alters endothelial angiogenic function and cell morphology
In vitro angiogenesis is impaired in uPAR-/- ECs: (A) WT cells on cytodex beads and embedded in a fibrin matrix were stained for actin using phalloidin-TRITC (red) and DNA (blue) and imaged using a 100× objective. After 24 hr incubation the bead was surrounded by adherent cells which were elongated. (B) Enhanced elongation of sprouts was observed from uPAR-/- ECs adherent on the beads and these sprouts appeared to be thinner. (C) Sprout elongation by WT cells in the presence of 10 ng/ml of VEGF. Boxed area shows the development of robust lamellipodia formation of WT cells. (D) Elongation process after 24 hr for uPAR-/- ECs in the presence of 10 ng/ml of VEGF. Boxed area shows that the sprouts derived from the uPAR-/- ECs are thinner and show truncated lamellipodia formation.