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Figure 6 | Journal of Angiogenesis Research

Figure 6

From: Methylnaltrexone Potentiates the Anti-Angiogenic Effects of mTOR Inhibitors

Figure 6

The effects of mTOR, Akt, Src, Rictor and PI3 kinase on VEGF-induced human EC proliferation and migration. Panel A: Bar graph representation of human EC assayed for VEGF (100 nM)-induced proliferation (24 hours) in the presence or absence of 48 hour pretreatment with mTOR siRNA, Akt siRNA, Src siRNA, Rictor (mTOR Complex 2 component) siRNA or pretreatment (1 hour) with LY294002 (PI3 kinase inhibitor, 10 μM). Experiments were performed in triplicate. Error bars = standard deviation. Panel B: Bar graph representation of human EC assayed for VEGF (100 nM)-induced migration (24 hours) in the presence or absence of 48 hour pretreatment with mTOR siRNA, Akt siRNA, Src siRNA, Rictor (mTOR Complex 2 component) siRNA or pretreatment (1 hour) with LY294002 (PI3 kinase inhibitor, 10 μM). Experiments were performed in triplicate. Error bars = standard deviation. Panel C: Bar graph representation of human EC assayed for VEGF (100 nM)-induced proliferation (24 hours) in the presence or absence of 48 hour pretreatment with Src or FKBP12 siRNA with addition of 100 nM MNTX and/or 10 nM temsirolimus. Experiments were performed in triplicate. Error bars = standard deviation. Panel D: Bar graph representation of human EC assayed for VEGF (100 nM)-induced migration (24 hours) in the presence or absence of 48 hour pretreatment with Src or FKBP12 siRNA with addition of 100 nM MNTX and/or 10 nM temsirolimus. Experiments were performed in triplicate. Error bars = standard deviation.

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