Figure 2
From: Methylnaltrexone Potentiates the Anti-Angiogenic Effects of mTOR Inhibitors
Comparison of MNTX and naltrexone on synergy with temsirolimus on VEGF-induced angiogenic events. Panel A: Bar graph representation of human EC assayed for VEGF (100 nM)-induced proliferation (24 hours) in the presence or absence of 10 nM MNTX, 100 nM MNTX, 10 nM naltrexone, 10 nM temsirolimus, 100 nM morphine + 10 nM temsirolimus, 10 nM MNTX + 10 nM temsirolimus, 100 nM MNTX + 10 nM temsirolimus or 10 nM naloxone + 10 nM temsirolimus. Experiments were performed in triplicate. Error bars = standard deviation. Panel B: Bar graph representation of human EC assayed for VEGF (100 nM)-induced migration (24 hours) in the presence or absence of 10 nM MNTX, 100 nM MNTX, 10 nM naltrexone, 10 nM temsirolimus, 100 nM morphine + 10 nM temsirolimus, 10 nM MNTX + 10 nM temsirolimus, 100 nM MNTX + 10 nM temsirolimus or 10 nM naloxone + 10 nM temsirolimus. Experiments were performed in triplicate. Error bars = standard deviation.