Figure 1
From: Chloride intracellular channel 1 functions in endothelial cell growth and migration
Establishing human endothelial cell lines with CLIC1 knockdown. A. Immunoblotting with polyclonal rabbit anti-CLIC1 antibody confirmed reduced CLIC1 protein expression in CLIC1 knockdown (C1 kd) endothelial cell lines. Immunoblotting with rabbit polyclonal anti-CLIC4 antibody verified that CLIC4 expression was not altered by clic1-targetting shRNA. Alpha-tubulin served as a loading control. B. Established HUVEC control and CLIC1 knockdown lines exhibited no gross morphological differences when cultured as a subconfluent monolayer on collagen-coated dishes. Scale bar, 30 μm.