Figure 2

Increased microscopic necrosis and bleeding in CIB1-KO allograft tumors. Fourteen days after tumor cell injection, tumors were isolated from animals and fixed. Sections were sectioned and stained with H&E or Masson's trichrome (MT) as described in Methods. Representative images were captured at two different magnifications using a Nikon D100 camera attached to a Nikon inverted microscope. Representative low magnification images (a, d, g, and j), and high magnification (b, c, e, f, h, i, k, and l) of melanoma and carcinoma tumors. Areas of necrosis (N) and bleeding are noted on H&E-stained sections (a, b, d, e, g, h, j, and k). Fibrosis stained in blue (identified with yellow arrows) is noted on MT-stained sections (c, f, i, and l).