Figure 2

The effects of R1-mAb and R2-mAb on the maintenance of EC structure of rats with Gal-N+LPS-induced acute hepatic failure. A: Representative features of the effects of R1-mAb and R2-mAb on maintenance of the SEC structure after intoxication by combination of Gal-N+LPS. The R1-mAb and R2-mAb treatment significantly augmented the destruction of the SEC architecture in the liver after Gal-N+LPS intoxication. The SEC architecture was evaluated by the ICAM-1-immunopositive vessels. Original magnification: × 40. B: Semi-quantitative analysis of the SEC architecture in the liver following the Gal-N+LPS intoxication. Individual treatment with R1-mAb and R-2mAb significantly augmented the destruction of the SEC architecture in the liver both at 24 h after intoxication. The destruction magnitude of R2-mAb was significantly higher than that of R-1 mAb. Cont: Control IgG-treated mice (G1). Mice in group 2 (G2: Gal-N) simultaneously received Gal-N (375 mg/kg), LPS (50 μg/kg), and the control IgG intraperitoneally at 0 h. Instead of the control IgG, animals in group 3 (G3: R1) and group 4 (G4: R2) received equal amounts of R1mAb or R2mAb intraperitoneally at 0 h, respectively. MV: microvessel. The data represent the mean ± SD. *: Statistically significant differences between the experimental groups (p < 0.05).