Fig. 1
Normal Angiogenesis in MK2-deficient mice. a The growing endothelial plexus. Isolectin-B4(IB4)-staining, quantification of the vascularized area (red line) relative to whole retina area (white line). Scale bar: 1 mm. b Angiogenic sprouts (red dots). IB4-staining, quantification per high power field (HPF). Scale bar: 200 μm. c Endothelial junctions. IB4-staining. Points were assigned according to the complexity of junctions (trifurcation 1 point, quattrofurcation 2 points, pentafurcation 3 points). Scale bar: 200 μm. d Vertical branching. IB4-staining. Analysis was done with a confocal microscope in order to count vessels penetrating the middle layer of the retina (left). Scale bar: 200 μm. e Pruning. IB4-negative and Collagen-IV-positive sleeves were counted in HPF. Scale bar: 100 μm. P indicates postnatal day. * = p < 0.05, ** = p < 0.01