Figure 9From: Embryonic rat vascular smooth muscle cells revisited - a model for neonatal, neointimal SMC or differentiated vascular stem cells?The effects of Notch inhibition with DAPT on Sox10 expression in A7r5 and A10 cells. A. Quiesced A10 cells were grown in media containing 10% FBS supplemented with or without 10 μM DAPT for 3 d before the expression of Sox10 was determined by immunocytochemistry. B. Relative gene expression of SMC markers [(SMA, MHC, CNN1 and smoothelin (SMO)] and neural stem cell MVSC markers [Sox10, Sox17 and S100β)] in A10 and A7r5 cells was determined by qRT-PCR. GAPDH was used to normalize gene expression. Data are mean ± SEM and are representative of three independent wells, *p < 0.01 when compared to 0.5% FCS.Back to article page