Figure 4
From: Notch and VEGF pathways play distinct but complementary roles in tumor angiogenesis
Combined Notch and VEGF blockaded disrupts tumor vasculature. (A) Immunofluorescence staining for Collagen IV (red) demonstrated vasculature (arrows) in areas of necrosis (N) as indicated by absence of nuclei by DAPI (blue), for NGP-N1D and NGP-N1D + BV. For clarity, a white dotted line delineates border between viable and necrotic tumor areas. In NGP-LacZ + BV arrowheads indicate coopted glomeruli. Bar = 200 μm. (B) Collagen IV was quantified, with NGP-LacZ + BV significantly higher in comparison to NGP-LacZ and NGP-N1D + BV. Error bars represent SD. *P < 0.05, **P < 0.01.