Figure 6

Changes in nuclear and cytosolic distribution of β-catenin in HUVECs: HUVECs were maintained in culture for different time intervals as described in the text. Medium was removed, the cells were harvested, fractionated and nuclear and cytosol were isolated. Protein equivalent amount of the cytosolic and nuclear fractions were subjected to SDS PAGE and western blot analysis with anti β-catenin antibody as described in the text. Lane1-4, 1^st -4^th day (A). The intensity of the immunoblotted bands were measured and expressed in intensity units/mm² (B). Percentage distribution of β-catenin in the nucleus and cytosol (C) * Statistically significant when compared to their relative levels in nucleus and cytosol on first two days p < 0.05.