Figure 1
From: Changes in expression of VE-cadherin and MMPs in endothelial cells: Implications for angiogenesis
Production of MMP-2 and MMP-9 by HUVECs: Equivalent aliquots of the medium normalized to the total cell protein from the cultures of HUVECs maintained in MCDB 131 medium collected at intervals of 24 hrs were subjected to zymography (A) and ELISA (C) as described in text. Lanes 1-5 1st-5th day. The activity of MMP-9 and MMP-2 in zymogram was quantitated using Quantity One 4.5.0 software BioRad geldoc and expressed in arbitrary units of intensity/mm2/mg protein (B). Values given are the average of intensity measurement of 6 experiments ± SEM. The protein level expression quantitated for MMP-9 and MMP-2 by ELISA using specific antibody (C). Values given are the average of duplicate analysis of 5 experiments ± SEM. * Statistically significant compared to the production on first day p < 0.05.